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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.121 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠甲基CpG结合蛋白2(MECP2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse MECP2(Methyl CpG Binding Protein 2) ELISA Kit |
| 别名 | RTS; AUTSX3; MRX16; MRX79; PPMX; RTT; Rett Syndrome; Mental Retardation,X-linked 16 |
| 货号 | ELK6723 |
| 反应种属 | Mouse |
| Q9Z2D6 | Q9Z2D6 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.121 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MECP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MECP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MECP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MECP2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science; |

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文献和实验清楚,因此其需要后续进一步分析确定,此外,RLGS图谱不能完全确认所缺失的片段是由于甲基化所致还是由于DNA本身缺失所致[5]且结果分析复杂,不易解释。 2.3.2 MBD(Methyl-CpG binding domain column chromatography,甲基化结合区)柱层析法 根据MBD蛋白家族和MeCP2的特性,Masahiko Shiraishi等2004年[50]提出了一种新的方法MBD柱层析法,用于筛选和发现基因组中甲基化的情况。并且比较了MBD和重亚硫酸盐基因组测序法,得出:用MBD
Evaluation of Rett Syndrome Symptom Improvement by Metabolic Modulators in Mecp2‐Mutant Mice
recapitulate many symptoms of Rett Syndrome, an X?linked disorder caused by mutations in methyl?CpG?binding protein 2 (MECP2 ). The study of Mecp2 ?null male mice has provided insight into pathogenesis of the disorder?most recently, dysregulation of cholesterol
Diabetes | 华科黄昆 / 武大郑凌团队揭示甲基化结合蛋白可调控脂肪细胞棕色化控制食物诱导的肥胖
Knockout of Mecp2 Upregulates Slpi to Reduce Obesity by Enhancing Browning》。该研究揭示了甲基化 CpG 岛结合蛋白一种非神经性功能机制,为肥胖和代谢紊乱提供新的潜在治疗靶标。黄昆教授主要从事代谢疾病机理及相关创新药物研究,有着长期综合利用多种生物学手段研究疾病病变机理及相关治疗药物的经历。郑凌教授主要研究糖尿病及其并发症,神经退行性疾病相关内容。图片来源:Diabetes具体内容作者首先分析了肥胖人群和高脂料饲养小鼠体内 Mecp2
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