小鼠谷氨酸半胱氨酸连接酶催化亚基(GCLC)酶联免疫吸附检测
试剂盒【Mouse GCLC(Glutamate Cysteine Ligase, Catalytic) ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.114 ng/mL
- 武汉
- ELK6361
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度：
0.114 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 小鼠谷氨酸半胱氨酸连接酶催化亚基(GCLC)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse GCLC(Glutamate Cysteine Ligase, Catalytic) ELISA Kit |
| 别名 | GCS; GLCL; GLCLC; Gamma-glutamylcysteine synthetase; Gamma-ECS |
| 货号 | ELK6361 |
| 反应种属 | Mouse |
| P97494 | P97494 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.114 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GCLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GCLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GCLC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GCLC in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |
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文献和实验Assessment of Glutathione Homeostasis
redox state. High levels of GSH are present in the central nervous system, particularly in astrocytes. GSH is synthesized into two enzymatic steps, the first, and the rate-limiting one, is catalyzed by glutamate–cysteine ligase (GCL) to form a dipeptide
Methods to Detect Protein Glutathionylation
Backos, D.S., Fritz, K.S., Roede, J.R., Petersen, D.R., and Franklin, C.C. 2011. Posttranslational modification and regulation of glutamate‐cysteine ligase by the alpha,beta‐unsaturated aldehyde 4‐hydroxy
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