- ￥1960 - 2800
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.116 ng/mL
- 武汉
- ELK5815
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Horse
- 样本：
Serum, plasma and other biological fluids.
- 灵敏度：
0.116 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 马基质金属蛋白酶7(MMP7)酶联免疫吸附检测试剂盒 |
| 英文名称 | Horse MMP7(Matrix Metalloproteinase 7) ELISA Kit |
| 别名 | MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase |
| 货号 | ELK5815 |
| 反应种属 | Horse |
| 检测类型 | Sandwich |
| 灵敏度 | 0.116 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Serum, plasma and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse MMP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse MMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse MMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse MMP7 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Tumor immunity; |
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文献和实验v468 Chapter 9 自身启动子报告基因验证转录靶点
, whereasclones carrying the correct insert from the MMP7 promoterwill produce an amplicon of 1,028 bp (see Note 12 ). 4. Prepare plasmid DNA from a positive clone from the masterplateby using the miniprep kit from Qiagen and subject it tosequencing reaction
质表达无影响；E2对hOB MMP-2及TIMP-1 蛋白质表达无影响；E2对人成骨肉瘤MG-63细胞株和hOB MMP-2活性亦无影响。结论：雌激素不足可导致其对人成骨样细胞MMP-1的抑制作用减弱，促进骨吸收和骨基质分解，此可能为雌激素缺乏致骨质疏松的重要发病机制。四、雌二醇对人成骨肉瘤MG-63细胞株和成人成骨细胞膜型 基质金属蛋白酶-1的影响目的：膜型基质金属蛋白酶-1（membrane type 1-matrix metalloproteinase, MT1-MMP）是最近发现的一种可由
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