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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.112 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 大鼠核因子κB亚基p105(NFKB-p105)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat NF-κB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit |
| 别名 | NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B |
| 货号 | ELK5691 |
| 反应种属 | Rat |
| Q63369 | Q63369 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.112 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验大鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫分析
大鼠核因子- κB 亚基p65 亲和肽(NF- κB p65) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中 核因子- κB 亚基p65 亲和肽(NF- κB p65) 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中 大鼠 核因子- κB 亚基p65 亲和肽(NF- κB p65) 的水平。用纯化的大鼠 核因子- κB 亚基p65
Activation of Nuclear Factor-B (NF-B) in Mononuclear Cells (MNC)
The transcription factor NF-κB was first identified as a regulator of the expression of the kappa light-chain gene in murine B lymphocytes (1 ). It has a central role in the immunological processes and regulates a wide range of genes
The nuclear factor-kappa B (NFκB) family of transcription factors has emerged as a signaling pathway that figures prominently in a cell’s initial response to a plethora of inflammatory stimuli. Modified lipids, oxidative stress, bacterial
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