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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Pig
- 样本:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
5.9 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 猪内皮型一氧化氮合酶(NOS3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Pig NOS3(Nitric Oxide Synthase 3, Endothelial) ELISA Kit |
| 别名 | ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS |
| 货号 | ELK5617 |
| 反应种属 | Pig |
| Q28969 | Q28969 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.9 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig NOS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig NOS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig NOS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig NOS3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Cardiovascular biology; |

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文献和实验-dependent and constitutively expressed and produce low levels of NO: NOS1 (neuronal NOS or nNOS), which is found mostly in neurons and skeletal muscle, and NOS3 (endothelial NOS or eNOS), which is found mostly in endothelial cells. NOS1 is critical
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