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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.126 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人凋亡抑制因子5(API5)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human API5(Apoptosis Inhibitor 5) ELISA Kit |
| 别名 | MIG8; FIF; AAC11; API5L1; XAGL; Cell migration-inducing gene 8; Antiapoptosis clone 11; Fibroblast Growth Factor 2-Interacting Factor 2; Migration-Inducing Protein MIG8 |
| 货号 | ELK5117 |
| 反应种属 | Human |
| Q9BZZ5 | Q9BZZ5 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.126 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human API5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human API5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human API5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human API5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Apoptosis; |

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文献和实验细胞。 LXMSUNLIGHT: 1、流式细胞术检测细胞凋亡率 2、AO/EB荧光染色 (Wang CY, James E, Jim L. Spontaneous apoptosis in human colon tumor cell lines and the relation of WT P53 to apoptosis. Chinese Medical Journal, 1996;109:537-541) 方法略有改进:将处于对数生长期的2株细胞制成细胞悬液,调成1×109/L细胞密度加入细胞培养瓶,待细胞
【求助】本人最近想研究某药物对凋亡调控的机理,如何筛查出该药物相关的作用通路???
,工作量就会大大降低。另外如果有钱的话,还可以考虑试试Cell Signaling的PathScan® Apoptosis Multi-Target Sandwich ELISA Kit #7105 (详见 http://www.cellsignal.com/products/7105.html) 这样就可以同时检测多个参与Apoptosis的关键分子,这个Kit可大大降低你的工作量。 good luck! carl97 非常感谢,我再试试
细胞术已经成为凋亡分析的重要检测手段。PharMingen公司提供了许多从多种角度研究凋亡的试剂系统,包括小鼠、大鼠和人类蛋白抗体:Fas和Fas配体、TRAIL和DR3/DR4、Bcl-2家族、Caspase酶家族及其它信号蛋白;再有,提供了多种流式细胞仪检测试剂盒,包括:Caspase-3 Assay Kit、APO-BRDU和APO-DIRECT Kit、Annexin V-FITC Apoptosis Detection Kit;另外,还提供了mRNA分析试剂——RiboQuant Rnase
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