- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.057 ng/mL
- 武汉
- ELK4503
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.057 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人Ras同源基因家族成员A(RHOA)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human RHOA(Ras Homolog Gene Family, Member A) ELISA Kit |
| 别名 | ARH12; ARHA; RHO12; RHOH12; Rho cDNA clone 12 |
| 货号 | ELK4503 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 0.057 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RHOA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RHOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RHOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RHOA in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity; |
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文献和实验Assessment of the Role for Rho Family GTPases in NADPH Oxidase Activation
Rac, a member of the Rho family small GTPases, plays a crucial role in activation of Nox family NADPH oxidases in animals, enzymes dedicated to production of reactive oxygen species such as superoxide. The phagocyte oxidase Nox2, crucial
In vitro Reconstitution of Activation of PLC by Ras and Rho GTPases
fusion protein in E. coli. Subsequently, we describe the methodology necessary to reconstitute this protein with K-Ras-4B and RhoA GTPases and measure its activation.
Multiplex Imaging of Rho Family GTPase Activities in Living Cells
. Here, we take as an example a combination of the RhoA biosensor based on a CFP/YFP FRET modality and the Cdc42 biosensor based on organic dyes that change fluorescence as a function of the local solvent polarity. We list the required optical components as well as cellular
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