- ￥1820 - 2600
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.112 ng/mL
- 武汉
- ELK4442
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
0.112 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人DNA指导聚合酶γ1(POLg1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human POLg1(Polymerase DNA Directed Gamma 1) ELISA Kit |
| 别名 | PEO; POLG1; POLGA; SANDO; SCAE; Mitochondrial DNA polymerase catalytic subunit |
| 货号 | ELK4442 |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 0.112 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human POLg1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POLg1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human POLg1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POLg1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |
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文献和实验Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease
DNA polymerase γ (pol γ) is the only DNA polymerase within the mitochondrion and is thus essential for replication and repair of mtDNA. POLG , the gene encoding the catalytic subunit of pol γ, is a major locus for a wide spectrum
ELATNESCLASRETSFITNG HCLASGKTSF MTTLCLRSIY EDLKMYHVEF QAMNAKLLMDPKRQIFLDQN MLAAIAELMQ ALNFDSETVP QKPSLKELDF YKTKVKLCILLHAFRIRAVT IDRMMSYLSS S2, FeaturesDL-IL12A-Ra大鼠白介素 12A （IL12A） 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定大鼠血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL
。非均相 EIA 需先进行游离的和结合的标记物的分离。如前所述，固相载体可用作一种分离手段。这种固相酶免疫测定方法在 1971 年最初建立时称为酶联免疫吸附剂测定（enzymelinked immunosorbent assay），简称 ELISA，在国内有译作酶联免疫吸附试验的，虽然含义不完全确切，但已习用。
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