小鼠促微管蛋白聚合蛋白(TPPP)酶联免疫吸附检测试剂盒【M
ouse TPPP(Tubulin Polymerization Promoting Protein) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.051 ng/mL
- 武汉
- ELK2645
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Mouse
- 样本：
Tissue homogenates and other biological fluids.
- 灵敏度：
0.051 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 小鼠促微管蛋白聚合蛋白(TPPP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse TPPP(Tubulin Polymerization Promoting Protein) ELISA Kit |
| 别名 | TPPP1; p25alpha; p25; TPPP/p25; Brain Specific Protein p25 Alpha; 25 kDa brain-specific protein |
| 货号 | ELK2645 |
| 反应种属 | Mouse |
| Q7TQD2 | Q7TQD2 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.051 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TPPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TPPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TPPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TPPP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science;Developmental science; |
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文献和实验, the more concentrated the peak of elution of tubulin protein. DAY 2: Cycling preparation of MT protein. Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport back to the laboratory.
per hour collecting 0.5 - 1 mL fractions. The first eluate is purified tubulin protein. Do not mix with later peak fractions, which contain microtubule-associated proteins (MAPs). Pool the purified tubulin +/- MAPs, divide into 0.5 mL aliquots, freeze
of tubulin protein. DAY 2: Cycling preparation of MT protein. Keep the brains in an evacuated plastic ziplock bag buried in ice from the time of slaughter during transport back to the laboratory. In a 4oC cold room, carefully and thoroughly
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