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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.073 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人钙调素(CAM)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human CAM(Calmodulin) ELISA Kit |
| 别名 | Calcium Modulated Protein |
| 货号 | ELK2014 |
| 反应种属 | Human |
| P0DP23 | P0DP23 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.073 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAM in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Metabolic pathway;Apoptosis;Infection immunity; |

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文献和实验实验28 真核生物钙调素的酶联免疫测定法 原理 本法是一种测定抗体的竞争性固相酶联免疫测定法(enzyme linked immunosorbent assay,ELISA)。先将抗原──CaM与固相载体(聚苯乙烯微量滴定板)结合,然后将经待检CaM(标准样品或检样)部分中和的兔抗CaM抗体加入微量滴定板孔中,抑制固相CaM和抗体的反应。接着,废弃上清液,洗涤固相表面,加入辣根过氧化物酶标记的葡萄
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ELISA KIT & ANTIBODY OF USCNLIFE
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