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文献和实验Studies of Calmodulin-Dependent Regulation
Methods are presented for purifying bovine testes calmodulin and the calmodulin-regulated plasma-membrane calcium ATPase from human erythrocytes by calcium dependent affinity chromatography. The assay of CaM Kinase II using a synthetic
A Microtiter-Plate Assay of Human NOS Isoforms
isozymes are highly regulated by Ca2+ and calmodulin (CaM), whereas the iNOS has CaM tightly bound. NOS are heme proteins that also contain tightly bound flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) and require tetrahydrobiopterin (BH
Cloning and Expression of Human Inducible Nitric Oxide Synthase
was then inserted back into oocytes to confirm functional expression. Nitrite production did not require the presence of Ca2+ and was not inhibited by trifluoperazine, a calmodulin (CaM), and brain-NOS inhibitor.
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