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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.054 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人脂肪分化相关蛋白(ADRP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human ADRP(Adipose Differentiation Related Protein) ELISA Kit |
| 别名 | PLIN2; ADFP; Adipophilin; Perilipin 2 |
| 货号 | ELK1534 |
| 反应种属 | Human |
| Q99541 | Q99541 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.054 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADRP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway;Cardiovascular biology; |

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文献和实验Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques
lipid bodies within leukocytes by light microscopy. These techniques include staining with osmium or use of different fluorescent probes such as Nile red, BODIPY, Oil red, P96 and immunofluorescence labeling for adipose differentiation-related protein
【原创】Protein A Sepharose 之进化篇。。。
[img][/img]Protein A 柱之进化 Protein A是一种金黄色葡萄球菌细胞壁蛋白质,能特异性地与人和哺乳动物抗体(主要是IgG)的Fc区结合。因而,将Protein A 与琼脂糖凝胶以一定的方式结合,可制备用于抗体纯化的亲和填料。 早期的Protein A柱结合的都是天然Protein A。天然Protein A由5个IgG结合域和其它未知功能的非Fc结合域组成,分子量约42KD,结构如图1所示。这种柱子对IgG的亲和能力很强,可以吸附大量的lgG。但同时,天然
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., Vukicevic, S., and Luyten, F.P. (1996) Primary structure and tissue distribution of Frzb, a novel protein related to Drosophila frizzled, suggest a role in human skeletal morphogenesis. J. Biol. Chem . 271 , 26131–26137. 6. Finch, P.W., He., X., Kelley, M
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