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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.059 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 大鼠胱天蛋白酶3(CASP3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat CASP3(Caspase 3) ELISA Kit |
| 别名 | CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1 |
| 货号 | ELK1528 |
| 反应种属 | Rat |
| P55213 | P55213 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.059 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CASP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CASP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CASP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Infection immunity; |

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文献和实验CUSABIO月度文献盘点:5月新增200篇产品引用文献,总文献数量已达 31200篇!
因子:14.1 发表期刊:Advanced Science CUSABIO助力产品: Mouse Interleukin 1β,IL-1β ELISA Kit; CSB-E08054m 研究亮点 早发型子痫前期患者胎盘滋养细胞中CASP3激活并裂解GSDME,导致细胞从凋亡转向焦亡,释放炎症因子。焦亡的滋养细胞进一步诱导M1型巨噬细胞极化,形成正反馈加剧炎症。动物模型中敲除Gsdme可减轻全身炎症。维生素D能抑制GSDME激活,为防治提供新靶点。 03
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,平均批间变异系数为11.4%;液相芯片与ELISA法检测结果的相关系数R2为0.88~0.99。 国外多家公司已推出了商品化的细胞因子液相芯片检测试剂盒,比如R&D 公司的Flurokine MAP系列、Linco Research公司的LINCOplex 试剂盒、BioSource公司的Antibody Bead Kit、Bio Rad公司的Bio Plex系列等,可用于来自人、小鼠、大鼠的样品,既有一次检测单种细胞因子的试剂盒,也有预先混合多种细胞因子抗体交联微球而一次检测多个指标
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