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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
6.25-400 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Cattle
- 样本:
serum, plasma and other biological fluids
- 灵敏度:
2.84 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 牛肌肉生长抑制素(MSTN)酶联免疫吸附检测试剂盒 |
| 英文名称 | Cattle MSTN(Myostatin) ELISA Kit |
| 别名 | GDF8; Growth Differentiation Factor 8 |
| 货号 | ELK11439 |
| 反应种属 | Cattle |
| O18836 | O18836 |
| 检测类型 | Sandwich |
| 灵敏度 | 2.84 ng/mL |
| 标准品 | 400 ng/mL |
| 检测范围 | 6.25-400 ng/mL |
| 样本类型 | serum, plasma and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle MSTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle MSTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle MSTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle MSTN in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Metabolic pathway;Bone metabolism; |

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文献和实验人肌肉生长抑制素(MSTN ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,组织及相关液体样本中 肌肉生长抑制素( MSTN ) 的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人肌肉生长抑制素( MSTN ) 水平。用纯化的人肌肉生长抑制素( MSTN ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入肌肉生长抑制素
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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