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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
3.11 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人白细胞激活黏附因子(ALCAM)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human ALCAM(Activated Leukocyte Cell Adhesion Molecule) Microsample ELISA Kit |
| 别名 | CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule |
| 货号 | ELK10263MS |
| 反应种属 | Human |
| Q13740 | Q13740 |
| 检测类型 | Sandwich |
| 灵敏度 | 3.11 ng/mL |
| 标准品 | 1000 ng/mL |
| 检测范围 | 15.63-1000 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALCAM in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | CD & Adhesion molecule;Tumor immunity; |

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文献和实验Leukocyte-Endothelial Monolayer Adhesion Assay (Static Conditions)
-receptor pairing of a series of adhesion molecule expressed by both circulating leukocytes and the endothelial cell (1 ). The sequential and coordinated interaction of these leukocyte-endothelial adhesion molecules, in conjunction with the localized
Heterotypic Cell Adhesion Assay for the Study of Cell Adhesion Inhibition
CD2 is a cell adhesion molecule that mediates T-cell activation by binding to its ligand CD58 on antigen-presenting cells. Interaction between CD2 and CD58 or leukocyte function-associated antigen-3 (LFA-3) helps to optimize immune
for analysis of blood cell (e.g., leukocyte) adhesion to endothelial cells, to each other, or extracellular matrix proteins. This chapter describes two basic protocols, one for analysis of cell adhesion under static conditions and the other for measurement
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