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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids
- 灵敏度:
0.06 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人转录因子ERG(ERG)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human ERG(Transcriptional regulator ERG) ELISA Kit |
| 别名 | Transcriptional regulator ERG |
| 货号 | ELK0673 |
| 反应种属 | Human |
| P11308 | P11308 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.06 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERG in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验为一个复合的电反应。引导的方法是将一个引导电极与角膜接触,将另一个面积较大的参照电极放在额部,当给视网膜以光刺激时,可在示波器上记录到一系列电变化,即视网膜电图。视网膜电图可分成几个部分,一个典型的视网膜电图如附图中的粗线所示。光刺激下,开始有一个小的负波(角膜为负),称 a波,然后出现一个正(角膜为正)的 b波。如刺激强度较大,则在 b波之后,还有一个上升较缓慢的正波(角膜为正),称 c波。在光刺激结束时,还会有一个角膜为正的向上突起,称 d波。据分析, a波主要
Functional Phenotyping of Mouse Models with ERG
processing, and during the follow-up of therapeutic interventions. Full-field electroretinography (ERG), yielding a sum response of event-related transient electrical activity of the entire retina to light stimulation, is widely applied in human
亦称视网膜动作电位图。网膜受光照时产生电位,把这种电位记录下来便是视网膜电位图,简称ERG。勿从杜波依斯-雷蒙(E.H.Dubois-Reymo-nd,1849)发现网膜静止电流和霍姆格伦(F.Holm-grem,1866)发现动作电流以来,为了探讨网膜的视觉机制把ERG作为有用的客观指标,对哺乳类、两栖类、爬行类、鱼类、头足类等动物进行了广泛的研究。把网膜放在黑暗中发现,产生的静止电位的极性是:脊椎动物在角膜侧为正,而头足类和其它少数无脊椎动物在巩膜侧为正,当网膜受光照时
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