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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.19 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 大鼠GATA结合蛋白2(GATA2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat GATA2(GATA Binding Protein 2) ELISA Kit |
| 别名 | Endothelial transcription factor GATA-2 |
| 货号 | ELK0558 |
| 反应种属 | Rat |
| Q924Y4 | Q924Y4 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.19 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GATA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GATA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GATA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GATA2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Tumor immunity; |

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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
法(ELISA)核小体测定 凋亡细胞的DNA断裂使细胞质内出现核小体。核小体由组蛋白及其伴随的DNA片断组成,可由ELISA法检测。 (一)检测步骤 1、将凋亡细胞裂解后高速离心,其上清液中含有核小体; 2、在微定量板上吸附组蛋白体; 3、加上清夜使抗组蛋白抗体与核小体上的组蛋白结合; 4、加辣过氧化物酶标记的抗DNA抗体使之与核小体上的DNA结合; 5、加酶的底物,测光吸收制。 (二)用途 该法敏感性高,可检测5*100/ml个凋亡细胞。可用于人、大鼠、小鼠的凋亡检测。该法不需要特殊仪器
竞争ELISA检测试剂盒,无需对样品中的cAMP进行乙酰化。 http://www.neweastbio.com/NonAcetylatedCamp.do 小猪亨亨 http://www.mediomics.com/camp_desinger.html 我准备试试这个 neweastbio 如何定量检测cAMP和cGMP
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