人线粒体外膜转位酶20(TOMM20)酶联免疫吸附检测试剂盒
【Human TOMM20(Mitochondrial Import Receptor Subunit TOM20 Homolog) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.052 ng/mL
- 武汉
- ELK0255
- 2026年04月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度：
0.052 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人线粒体外膜转位酶20(TOMM20)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human TOMM20(Mitochondrial Import Receptor Subunit TOM20 Homolog) ELISA Kit |
| 别名 | MOM19; MAS20; TOM20; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20 |
| 货号 | ELK0255 |
| 反应种属 | Human |
| Q15388 | Q15388 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.052 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TOMM20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TOMM20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TOMM20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TOMM20 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |
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文献和实验Identification of Motifs in Protein Sequences
, O. 1994. Targeting of membrane proteins to endosomes and lysosomes. Trends Cell Biol. 4:292‐297. Schwarz, E. and Neupert, W. 1994. Mitochondrial protein import: Mechanisms
to Caenorhabditis elegans biology 68. YIDB http://www.EMBL-Heidelberg.DE/ExternalInfo/seraphin/yidb.html Yeast nuclear and mitochondrial intron sequences l Genetic and Physical Maps 69. DRESH http://www.tigem.it/LOCAL/drosophila/dros.html Human cDNA
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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