人周期素依赖性激酶抑制因子1A(CDKN1A)酶联免疫吸附检
测试剂盒【Human CDKN1A(Cyclin Dependent Kinase Inhibitor 1A) ELISA Kit】- ￥1960 - 2800
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.061 ng/mL
- 武汉
- ELK8797
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
tissue homogenates, cell lysates and other biological fluids
- 灵敏度：
0.061 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1960.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2800.0 |
| 中文名称 | 人周期素依赖性激酶抑制因子1A(CDKN1A)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human CDKN1A(Cyclin Dependent Kinase Inhibitor 1A) ELISA Kit |
| 别名 | WAF1; p21; CIP1; p21WAF1; CAP20; CDKN1; MDA-6; SDI1; p21CIP1; CDK-Interacting Protein 1; Melanoma differentiation-associated protein 6 |
| 货号 | ELK8797 |
| 反应种属 | Human |
| P38936 | P38936 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.061 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDKN1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDKN1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDKN1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDKN1A in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Developmental science; |
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文献和实验Analysis of Cyclin-Dependent Kinase Activity
are therefore needed to test chemical candidates for such therapy. Here we present a protocol for analysis of kinase activity for cyclin-dependent kinases using general substrates that will detect the broad activity of cyclin-dependent kinases (Fig. 1 ).
Measuring Cyclin-Dependent Kinase Activity
The activity of cyclin-dependent kinases (CDKs) lies at the heart of cell cycle and checkpoint control. These complexes have been called the “cell cycle engine,” as it is their enzymatic activity that pushes the cells through the cell
Assays for Cyclin-Dependent Kinase Inhibitors
Cyclin-dependent kinases (CDK) are serine/threonine kinases that regulate cell cycle control and progression (1 ,2 ). A CDK holoenzyme complex is active if associated with its cyclin partner and if the complex is phosphorylated at specific
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