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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
1.57-100 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.54 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 大鼠封闭蛋白16(CLDN16)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat CLDN16(Claudin 16) ELISA Kit |
| 别名 | PCLN1; HOMG3; Paracellin-1; Hypomagnesemia 3,With Hypercalciuria And Nephrocalcinosis |
| 货号 | ELK7269 |
| 反应种属 | Rat |
| Q91Y55 | Q91Y55 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.54 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CLDN16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CLDN16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CLDN16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CLDN16 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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