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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.057 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠过氧化物酶体增殖物激活受体δ(PPARd)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse PPARd(Peroxisome Proliferator Activated Receptor Delta) Microsample ELISA Kit |
| 别名 | FAAR; NR1C2; NUC1; NUCI; PPARB; PPARδ/B; Nuclear Receptor Subfamily 1,Group C,Member 2; Nuclear hormone receptor 1; Peroxisome proliferator-activated receptor beta |
| 货号 | ELK6907MS |
| 反应种属 | Mouse |
| P35396 | P35396 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.057 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PPARd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PPARd in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Metabolic pathway;Tumor immunity; |

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文献和实验crinology. First published April 3, 2003 as doi:10.1210/ me. 2002- 0410 Title:Peroxisome Proliferator-activated Receptor γ Inhibits Expression of Minichromosome Maintenance Proteins in Vascular Smooth Muscle Cells 过氧化物酶体增殖子激活受体γ(PPARγ)抑制血管平滑肌细胞内微型染色体维系蛋白
Analyzing PPAR/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry
for gaining insights into the conformational changes of the peroxisome proliferator-activated receptor alpha after binding of low-molecular weight ligands. Our strategy provides a basis to efficiently characterize target protein-drug
The capacity to induce the association of peroxisome proliferator-activated receptors (PPARs) with different transcriptional coregulators is determined by the peculiar 3D-structure that the receptors adopt when bound with a specific ligand
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