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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Tissue homogenates and other biological fluids.
- 灵敏度:
0.112 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 大鼠含缬酪肽蛋白(VCP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat VCP(Valosin Containing Protein) ELISA Kit |
| 别名 | IBMPFD; p97; TERA; Transitional Endoplasmic Reticulum ATPase; 15S Mg(2+)-ATPase p97 subunit |
| 货号 | ELK6826 |
| 反应种属 | Rat |
| P46462 | P46462 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.112 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VCP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science; |

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文献和实验The following video tutorial described how to run a sandwich ELISA, also known as enzyme-linked immunosorbent assay, using a general PeproTech protocol. 接下来的实验指导讲述PeproTech夹心法ELISA,即酶联免疫吸附试验的通用步骤 The capture, standard, and detection are supplied
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