- ￥1820 - 2600
- ELK Biotechnology
- 0.32-20 ng/mL
- 0.125 ng/mL
- 武汉
- ELK5091
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.32-20 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
0.125 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人间隙连接蛋白β6(GJb6)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human GJb6(Gap Junction Protein Beta 6) ELISA Kit |
| 别名 | HED; DFNA3; CX30; ED2; EDH; Ectodermal Dysplasia 2,Hidrotic; Connexin 30 |
| 货号 | ELK5091 |
| 反应种属 | Human |
| O95452 | O95452 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.125 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GJb6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GJb6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GJb6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GJb6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |
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文献和实验的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
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