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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.122 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人抗药蛋白(SRI)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human SRI(Sorcin) ELISA Kit |
| 别名 | SCN; CP-22; V19; 22 kDa protein |
| 货号 | ELK5034 |
| 反应种属 | Human |
| P30626 | P30626 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.122 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SRI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SRI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SRI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SRI in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验相关实验
研究。高度优化的ELISA检测试剂盒可以在检测标本时将干扰因素和非特异反应减少到最低水准,博士德生产的ELISA试剂盒是通过充分验证的, 敏感性:博士德的ELISA试剂盒使用标准的双抗体夹心技术,并且引进亲和素—生物素—过氧化物酶系统,以进一步提高敏感性。 稳定性:一般ELISA试剂盒所用的包被方法,很容易因干燥导致包被的抗体失去活性,从而影响结果的准确性。为此博士德进行了长期的研究,以期找到一种包被后抗体长期不失活的方法,甚至是在室温。博士德自主开发的新包被方法,正是采用了这一技术。博士
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人抗药蛋白(SRI)酶联免疫吸附检测试剂盒【Human SRI(Sorcin) ELISA Kit】
¥1820 - 2600





