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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.114 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人UDP-葡萄糖糖蛋白糖基转移酶1(UGGT1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human UGGT1(UDP-Glucose Glycoprotein Glucosyltransferase 1) ELISA Kit |
| 别名 | UGTR; HUGT1; GT; UGCGL1; UGGT; UGT1; UDP-glucose ceramide glucosyltransferase-like 1 |
| 货号 | ELK4813 |
| 反应种属 | Human |
| Q9NYU2 | Q9NYU2 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.114 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UGGT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UGGT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UGGT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UGGT1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |

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文献和实验酸和天冬氨酸以外的任意一种氨基酸)N--糖基化序列的特异天冬酰胺残基上开始,当新合成的蛋白质进入内质网腔后就发生 N 端序列糖基化反应(图 25-1B )。接着,当糖蛋白从内质网经高尔基体到达其最终目的地时,Glc3Man9GlcNAC2 前体沿着分泌途径进行进一步加工处理。如果糖基侧链能被内质网和高尔基体中的糖苷酶和糖基转移酶加工的话,Glc3Man9GlcNAc2 前体就会被成功转化为 Man9GlcNAc2 和 Man5GlcNAC2 范围内的髙甘露糖型 N-聚糖(图 25-1A), 以及复杂
Metabolic Labeling of Glycoproteins with Radioactive Sugars
for characterization of glycoprotein glycans from cultured cells. GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library Table
乙二醇甲基丙烯酸酯 GOD glucose oxidase 葡萄糖氧化酶 GP gold particle 金颗粒 HAV hepatitis A virus 甲型肝炎病毒 HBV hepatitis B virus 乙型肝炎病毒 HBV-DNA hepatitis B virus DNA 乙型肝炎病毒DNA HC Huntington's chorea 亨廷顿氏舞蹈症 HCG human chorionic
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