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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.111 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人Fission 1蛋白(FIS1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human FIS1(Fission 1) ELISA Kit |
| 别名 | CGI-135; TTC11; Tetratricopeptide Repeat Domain 11; Mitochondrial fission 1 protein |
| 货号 | ELK4182 |
| 反应种属 | Human |
| Q9Y3D6 | Q9Y3D6 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.111 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FIS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FIS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FIS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FIS1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity; |

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文献和实验Reliable Method for Detection of Programmed Cell Death in Yeast
and strains lacking the mitochondrial fission genes DNM1 /Drp1 and FIS1 , orthologs of mammalian cell death regulators. Cell viability following treatment with acetic acid is quantified by colony formation and vital dye (FUN1) staining to reproducibly detect
就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml,所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量,排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标,形式比较固定。为满足不同客户的实际需要和研究兴趣,BD于2005年初推出了CBA Flexset
的酶活力直接反映游离的酶标记物。均相EIA在临床检验中较少应用。非均相EIA需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在1971年最初建立时称为酶联免疫吸附剂测定(enzyme linked immunosorbent assay),简称ELISA,在国内有译作酶联免疫吸附试验或酶标,已习用。2、ELISA的原理和类型2.1. ELISA的原理ELISA的基础是抗原或抗体的固相化及抗原或抗体的酶标记。结合在固相载体表面的抗原或抗体仍保持其免疫
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