- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.05 ng/mL
- 武汉
- ELK4099MS
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates and other biological fluids.
- 灵敏度：
0.05 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人肝配蛋白A5(EFNA5)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human EFNA5(Ephrin A5) Microsample ELISA Kit |
| 别名 | AF1; EFL5; EPLG7; GLC1M; LERK7; RAGS; AL-1; EPH-related receptor tyrosine kinase ligand 7 |
| 货号 | ELK4099MS |
| 反应种属 | Human |
| P52803 | P52803 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.05 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EFNA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EFNA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA5 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Neuro science; |
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文献和实验Intracellular Immunofluorescent Staining for Flow Cytometry
for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines
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