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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
31.25-2000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
12.3 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人生长停滞特异性蛋白6(GAS6)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human GAS6(Growth Arrest Specific Protein 6) ELISA Kit |
| 别名 | AXLLG; AXSF; AXL Stimulatory Factor; AXL receptor tyrosine kinase ligand |
| 货号 | ELK3882 |
| 反应种属 | Human |
| Q14393 | Q14393 |
| 检测类型 | Sandwich |
| 灵敏度 | 12.3 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAS6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAS6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAS6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAS6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Immune molecule; |

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文献和实验酶免疫测定(enzyme immunoassay, EIA)或免疫酶技术(immunoenzymatic technique)是指用酶标记抗体或酶标记抗体进行的抗原抗体反应。它采用抗原与抗体的特异反应与酶连接,然后通过酶与底物产生颜色反应,用于定量测定。目前常用的方法称为酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA),其方法简单,方便讯速,特异性强。ELISA是由抗原(抗体)先结合在固相
by adding 100 µl of 1M Phosphoric Acid. Read at 450 nm. Conjugate Preparation 1. Centrifuge 0.3ml enzyme (alkaline phosphatase, Sigma type VII, sp act. 1,140 U/ml) at 8,000 x g for 2 mins. 2. Add to the pellet 0.1 ml specific antibody
ELATNESCLASRETSFITNG HCLASGKTSF MTTLCLRSIY EDLKMYHVEF QAMNAKLLMDPKRQIFLDQN MLAAIAELMQ ALNFDSETVP QKPSLKELDF YKTKVKLCILLHAFRIRAVT IDRMMSYLSS S2, FeaturesDL-IL12A-Ra大鼠白介素 12A (IL12A) 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定大鼠血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL
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