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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
5.3 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人凋亡诱导因子(AIF)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human AIF(Apoptosis Inducing Factor) ELISA Kit |
| 别名 | PDCD8; Programmed Cell Death protein 8; Apoptosis-inducing factor 1, mitochondrial |
| 货号 | ELK1670 |
| 反应种属 | Human |
| O95831 | O95831 |
| 检测类型 | Sandwich |
| 灵敏度 | 5.3 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AIF in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Apoptosis; |

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文献和实验和执行中非常重要的分子,但某些蛋白质从线粒体内膜腔释放而诱导的凋亡可以不通过caspase产生,称为caspase非依赖性通路。成熟型的丝氨酸蛋白激酶Omi(iV-名HtrA2),在凋亡发生时和Smac一起从线粒体释放到细胞质,一方面灭活1AP,释放caspase,对凋亡复合体起辅助作用,促进凋亡的发生(caspase依赖性通路);另一方面通过其丝氨酸酶活性来激活凋亡,称为caspase非依赖性通路。此外,凋亡诱导因子(apoptosis inducing factor,AIF)位于线粒体的内膜腔内
细胞色素c释放的效率远高于Bax。 线粒体释放的另一凋亡诱导因子是凋亡诱导因子(apoptosis inducing factor ,AIF),其分子量约49605,是一种黄素蛋白,正常生理状态下,AIF被封闭于线粒体中,在细胞发生凋亡时被转运入细胞核。重组的AIF能诱导细胞核中染色质凝集和DNA大规模降解。AIF还能诱导纯化的线粒体释放细胞色素c和caspase-9。
(Bax, Bak, Bok/Mtd, Bcl-rambo) into heterodimeric units or 'pores' in the outer mitochondrial membrane. This causes the release of numerous 'apoptogenic' factors, which include cytochrome c, Smac/DIABLO, apoptosis-inducing factor (Aif
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