人60kD热休克蛋白(HSPD1)酶联免疫吸附检测试剂盒【H
uman HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 1.57-100 ng/mL
- 0.59 ng/mL
- 武汉
- ELK1613
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
1.57-100 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度：
0.59 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人60kD热休克蛋白(HSPD1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit |
| 别名 | HSP60; GROEL; CPN60; HuCHA60; Heat Shock Protein 60; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein |
| 货号 | ELK1613 |
| 反应种属 | Human |
| P10809 | P10809 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.59 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSPD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSPD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSPD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSPD1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Tumor immunity; |
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文献和实验RT-PCR Quantitation of HSP60 mRNA Expression
Heat shock protein 60 (HSP60, HSPD1) is a “chaperonin” that facilitates folding of nascent proteins into proper conformations (1 ). It is thought to play a critical role in the assembly, folding, and transport of proteins in the mitochondria
酶联免疫吸附实验（ELISA）BIOX.CN 2004-9-7 14:41:36来源：生命经纬基本原理1971年Engvall和Perlmann发表了酶联免疫吸附剂测定（enzyme linked immunosorbent assay，ELISA）用于IgG定量测定的文章，使得1966年开始用于抗原定位的酶标抗体技术发展成液体标本中微量物质的测定方法。这一方法的基本原理是：①使抗原或抗体结合到某种固相载体表面，并保持其免疫活性。②使抗原或抗体与某种酶连接成酶标抗原或抗体，这种酶标抗原或抗体
一、基本原理1971年Engvall和Perlmann发表了酶联免疫吸附剂测定（enzyme linked immunosorbent assay，ELISA）用于IgG定量测定的文章，使得1966年开始用于抗原定位的酶标抗体技术发展成液体标本中微量物质的测定方法。这一方法的基本原理是：①使抗原或抗体结合到某种固相载体表面，并保持其免疫活性。②使抗原或抗体与某种酶连接成酶标抗原或抗体，这种酶标抗原或抗体既保留其免疫活性，又保留酶的活性。在测定时，把受检标本（测定其中的抗体或抗原）和酶标
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