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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.059 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人低氧诱导因子1α(HIF1a)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit |
| 别名 | HIF1-Alpha; HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 |
| 货号 | ELK1603 |
| 反应种属 | Human |
| Q16665 | Q16665 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.059 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIF1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIF1a in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Tumor immunity;Cardiovascular biology;Reproductive science; |

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