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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
7.82-500 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
2.8 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 小鼠粒细胞集落刺激因子(GCSF)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse GCSF(Colony Stimulating Factor 3, Granulocyte) ELISA Kit |
| 别名 | CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim |
| 货号 | ELK1026 |
| 反应种属 | Mouse |
| 检测类型 | Sandwich |
| 灵敏度 | 2.8 pg/mL |
| 标准品 | 500 pg/mL |
| 检测范围 | 7.82-500 pg/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GCSF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GCSF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GCSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GCSF in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Hematology; |

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文献和实验The hematopoietic growth factor granulocyte colony-stimulating factor (G-CSF) has been in the clinic since 1986, when the clinical development of bacterially synthesized recombinant filgrastim began (1 ). A glycosylated form of G-CSF
Acute myeloid leukemia (AML) has an annual incidence of 2.4 cases per 100,000 persons and is the most common form of acute leukemia in adults (1 , 2 ). AML is a model for drug-resistant human cancers, and current therapies for AML possess
Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms
With the completion of the first map of the entire human genome, it is estimated that there are approx 35,000 genes, which encode for translated RNA and protein products (1 ,2 ). The difference between any two human genomes is estimated
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