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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.115 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 大鼠B-细胞κ-轻肽基因增强子抑制因子激酶β(IkBKb)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat IkBKb(Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta) ELISA Kit |
| 别名 | IKK2; NFKBIKB; IKK-beta; IKKB; I-kappa-B kinase 2; Nuclear factor NF-kappa-B inhibitor kinase beta |
| 货号 | ELK10064 |
| 反应种属 | Rat |
| Q9QY78 | Q9QY78 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.115 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IkBKb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IkBKb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IkBKb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IkBKb in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |

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Dissociated Retina Immunohistochemistry
Solutions 100X trypsin 10 mg/ml in 1X PBS Sigma #T2271 100X soybean trypsin inhibitor 10 mg/ml in 1X PBS Sigma #T6522 1000X DNaseI 2mg/ml in HBSS Sigma #D4513 Explant Culture
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