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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.097 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 大鼠趋化因子C-X-C-基元受体4(CXCR4)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat CXCR4(Chemokine C-X-C-Motif Receptor 4) Microsample ELISA Kit |
| 别名 | CD184; Fusin; NPYR; HSY3RR; LAP3; LCR1; LESTR; NPY3R; NPYRL; WHIM; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3 |
| 货号 | ELK10056MS |
| 反应种属 | Rat |
| O08565 | O08565 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.097 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CXCR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CXCR4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity; |

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文献和实验Detailed understanding of chemokine action ultimately requires molecular cloning of chemokine receptor genes and cDNAs. Both forward and reverse genetic methods have been used for this purpose (1 ). Forward methods, which involve primary
Epigenetic Regulation of Chemokine/Chemokine Receptor Expression
for several members of the CXC (ELR+ ) chemokine family. In addition we describe a chromatin immunoprecipitation assay to determine the association between chromatin transcription markers and DNA following pretreatment of cell cultures with an HDi, Trichostatin A (TSA
Chemokine Receptor Antagonist Development
This chapter describes assays that focus on the characterization of compounds identified in high-�throughput screening campaigns, and the subsequent medicinal chemistry programs. They cover methods to determine potency
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