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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.14 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠视黄醇X受体β(RXRβ)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse RXRβ(Retinoic Acid Receptor RXR-beta) ELISA Kit |
| 别名 | RXRB; NR2B2; Retinoic acid receptor RXR-beta; Nuclear receptor subfamily 2 group B member 2; Retinoid X receptor beta |
| 货号 | ELK0510 |
| 反应种属 | Mouse |
| 检测类型 | Sandwich |
| 灵敏度 | 0.14 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RXRβ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RXRβ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RXRβ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RXRβ in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Metabolic pathway;Endocrinology;Hormone metabolism; |

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文献和实验Cell Rep Med:李振斐 / 黄盛松团队确认 3βHSD1 为晚期前列腺癌治疗新靶点
主要利用药物阿比特龙(abiraterone)和恩杂鲁胺(enzalutamide)来治疗 CRPC。 阿比特龙通过抑制代谢酶 CYP17A 来减少肾上腺合成脱氢表雄酮(dehydroepiandrosterone,DHEA);恩杂鲁胺通过与雄激素竞争性结合雄激素受体(androgen receptor,AR)来延缓前列腺癌进展。当这两个药物耐受后,患者几乎无药可用。 阿比特龙和恩杂鲁胺耐受后的前列腺癌异质性增加。一方面,雄激素非依赖性的神经内分泌型前列
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