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- 技术资料
- 库存:
100
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
1个/袋,2个/箱
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文献和实验/+/q0s8fX9J0KCtH7EtiZy60HdVrVJceMfUh+FwlIe3hgwd49eoVTp86Beu9+xASEgINDQ0YkEBSqWIlNGveHHXr1c1AWpKoNAK+vcZ3uqSqoUZiBuXBebIW4ybYo9rqu9jdLk+k/wd0TOPxh2rmhH5O/uiPSpFfKZoAvwMVTTwjjhf8FRc22cLHcBS6lzFGzn7VMWecDbbbTUD5+jnC82d4XxmEWv0d0XRqI7xbsxP2f1OB5m86D5vHamL
Large Scale Tubulin Preparation
30' at 40K in a 50.2Ti rotor at 4¡C. 10. Collect supernatant, measure concentration by Bradford and load onto the PC column (approx. 50 cm high X 5 cm ID = ~ 1L; Flow rate = 6 ml/min). After the sample is loaded and ~150 ml buffer has flowed
Large Scale Tubulin Preparation
should not be more than 25 mg/ml. Depolymerize on ice for 40' and then spin the depolymerization mix 30' at 40K in a 50.2Ti rotor at 4¡C. 10. Collect supernatant, measure concentration by Bradford and load onto the PC column (approx. 50 cm high X 5 cm ID = ~ 1L; Flow
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