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文献和实验CellTrace CFSE Cell Proliferation Kit
实验材料 Kit Contents CellTrace™ CFSE (Component A), 10 vials, each containing 50 μg of lyophilized powder DMSO (Component B), 1 vial containing 0.5 mL of high quality dimethylsulfoxide
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
solvents, lyophilization followed by permeabilization in acetone has been used successfully in place of aldehyde fixation for X-gal staining in C. elegans (Fire, 1992). The amount of ferricyanide and ferrocyanide can be varied. A more rapid
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