SIGMA 102466-5L 乙二醇 107-21-1

Purification of dnEBNA-1/Soft from E. coli BL21 LysS

Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (dnEBNA-1/Soft)in E.coli BL21 LysS per 0.5L LB+ ampicillin (grow two 0.5L cultures of each) Incubate ~2hrs 37℃250rpm until OD600 = 0.4-0.6 Induce with 5ml 100mM ITPG

肝刺激因子对人肝癌细胞BEL-7402 p21ras表达的影响

2+ DPBS洗涤3次, 换用无血清培养基(内含胰岛素10 μg/ml, 谷氨酰胺2 mmol/L)培养24 h。设4个实验组: (1)对照组, 不含HSS和EGF; (2) HSS组, 加HSS 50 μg/ml; (3) EGF组, 加EGF 20 ng/ml (rhEGF, 美国Promega公司); (4) HSS+EGF组, 加HSS 50 μg/ml和EGF 20 ng/ml。分别孵育6、 12、 24、 36和48 h后收集细胞并裂解。以Western blot法测定p21ras的表达

浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性

. Proc Natl Acad Sci U S A, 2004. 101(45): p. 15986-91. [20] Sotelo, J., et al., Long-range enhancers on 8q24 regulate c-Myc. Proc Natl Acad Sci U S A, 2010. 107(7): p. 3001-5. [21] Weinmann, A.S., Novel ChIP-based strategies to uncover transcription