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- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12 months
- 英文名:
Recombinant Human CISD1 Protein, N-GST & C-His
- 供应商:
武汉益普生物科技有限公司
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1268.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1868.0 |
| 规格: | 1mg | 产品价格: | ¥9764.0 |
| 产品名称 | Recombinant Human CISD1 Protein, N-GST & C-His |
| 货号 | HP104022 |
| 描述 | Recombinant Human CISD1 Protein, N-GST & C-His ( HP104022 ) expressed in E. coli , spanning Ala16-Thr108 . Purity: >90% by SDS-PAGE. Highlights His-Tagged — N-terminal 6×His tag for IMAC purification. E. coli Expression — High-yield, cost-effective production. High Purity — >90% purity verified by SDS-PAGE. |
| 应用实验 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 宿主/表达体系 | E. coli |
| 基因号 | Q9NZ45 |
| 状态 | Lyophilized |
| 蛋白质长度 | Ala16-Thr108 |
| 分子量 | 38.75 kDa |
| 纯度 | >90% as determined by SDS-PAGE. |
| 储存温度 | -20℃ |
| 别名 | C10orf70, CDGSH iron-sulfur domain-containing protein 1, CISD1, Cysteine transaminase CISD1, EC:2.6.1.3, MitoNEET, ZCD1 |

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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
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