- $1268 - 9764
- abinScience
- France
- PR129012
- 2026年04月22日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
twelve months
- 英文名：
Recombinant Arabidopsis thaliana CSN4 Protein, N-His
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | $1268.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | $1868.0 |
| 规格： | 1mg | 产品价格： | $9764.0 |
| 产品名 | Recombinant Arabidopsis thaliana CSN4 Protein, N-His |
| 货号 | PR129012 |
| 别名 | COP9 signalosome complex subunit 4, AtS4, Signalosome subunit 4, Constitutive photomorphogenesis protein 8, Protein FUSCA 4, CSN4, COP8, FUS4, At5g42970, MBD2.17 |
| 表达系统 | E. coli |
| Accession号 | Q8L5U0 |
| 种属 | Arabidopsis thaliana (Mouse-ear cress) |
| 背景 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 蛋白长度 | Asp2-Val397 |
| 预测分子量 | 46.06 kDa |
| 性质 | Recombinant |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 说明 | For research use only. |
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文献和实验A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thaliana
experimental samples in a high-throughput, reproducible fashion with minimal technical variability. This chapter describes a pipeline used for labeling living Arabidopsis thaliana plants with nitrogen-15 (15 N) and how this can be used, in conjunction
基因组中最小的。每个单倍染色体组（ n=5）的总长只有 7000万个碱基对，即只有小麦染色体组长的 1/80，这就使克隆它的有关基因相对说来比较容易。拟南芥是自花受粉植物，基因高度纯合，用理化因素处理突变率很高，容易获得各种代谢功能的缺陷型。例如用含杀草剂的培养基来筛选，一般获得抗杀草剂的突变率是 1/100000。由于有上述这些优点，所以拟南芥是进行遗传学研究的好材料，被科学家誉为“植物中的果蝇”。
ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
Nuclei isolation modified from Hamilton, Kunsch, and Temperli (1972) Anal. Biochem. 49:48-57; further modified by Tom Guilfoyle, then N. Olszewski and Eric Richards. Procedure: (all steps at 0-4C unless indicated) Harvest 100 g
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