- ￥1268 - 9764
- abinScience
- France
- MC412012
- 2026年08月30日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12 months
- 英文名：
Recombinant Mouse CKAP4 Protein, N-GST & C-His
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1268.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1868.0 |
| 规格： | 1mg | 产品价格： | ￥9764.0 |
| 产品名称 | Recombinant Mouse CKAP4 Protein, N-GST & C-His |
| 货号 | MC412012 |
| 描述 | Recombinant Mouse CKAP4 Protein, N-GST & C-His ( MC412012 ) expressed in E. coli , spanning Leu438-Ile575 . Purity: >90% by SDS-PAGE. Highlights His-Tagged — N-terminal 6×His tag for IMAC purification. E. coli Expression — High-yield, cost-effective production. High Purity — >90% purity verified by SDS-PAGE. |
| 应用实验 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 宿主/表达体系 | E. coli |
| 基因号 | Q8BMK4 |
| 状态 | Lyophilized |
| 蛋白质长度 | Leu438-Ile575 |
| 分子量 | 43.03 kDa |
| 纯度 | >90% as determined by SDS-PAGE. |
| 储存温度 | -20℃ |
| 别名 | p63, Climp-63, CKAP4, 63-kDa cytoskeleton-linking membrane protein, Cytoskeleton-associated protein 4 |
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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
5- If required, bring to 1pM RARE or any HRE you need 6- Incubate 30min at 4 _ C 7- Add 50_ L of the GST affinity matrix 8- Incubate for 2 hours at 4 _ C 9- Wash twice with PBS-DTT buffer (quick pulse to 500 x g) 10- Add 25_ L of SDS loading buffer directly
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