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文献和实验dilutions can be conveniently performed in each U‐bottom 96‐well microtiter plate. In this example, the wells in rows 1 and 5 are filled with the individual virus samples to be analyzed while the wells in rows 2 to 4 and 6 to 8 are each filled with 180 µl
96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测
Let sit at Rt for >15’ Suck off the medium of the 96 wells and add 100 ul of mixture (step 5e) by multichanel pipette. Let sit in incubator for >1.5 hours. Add 100 ul of D20F and let it grow for two days. Dual luciferase assay: Reagents:
considered a significant advance over traditional techniques. In fact, it is rapid, versatile, quantitative, and highly reproducible with a low intratest variation between datapoints (�15% SD); it is useful in a large-scale, antitumor drug-screening program
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