Oxidized (High) Protein Carbon

Protein Carbonyl Measurement by ELISA

prepared of serum albumin containing increasing proportions of hypochlorous acid (HOCl)- oxidized protein that is calibrated colorimetrically. This method requires only microgram quantities of protein and avoids the high and sometimes variable blanks owing

Quality Control Methodology for High-Throughput ProteinProtein Interaction Screening

, quality controls are required to deal with the inherent imperfect nature of experimental assays. In this chapter, we discuss experimental and statistical methodologies to quantify error rates in high-throughput protein–protein interactions screens.

Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles

and Analysis ). It may be necessary to include exogenous lipid during the solubilization step to stabilize the protein (2 ). Ideally, the chosen detergent should have a high critical micelle concentration to enable the detergent to be removed following