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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
Yu, X., Sainz, B. Jr. and Uprichard, S.L. 2009. Development of a cell‐based hepatitis C virus infection fluorescent resonance energy transfer assay for high‐throughput antiviral compound screening. Antimicrob. Agents Chemother. 53:4311‐4319
A Homogeneous Assay to Assess GABA Transporter Activity
a convenient functional uptake assay for GABA transport into cell lines transiently transfected with GABA transporter?1 (GAT?1) and other GAT isoforms. This facile, homogeneous assay allows for the determination of K m , V max , and K i values. The assay
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