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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
Phosphoamino acid analysis:Mark Kamps's method
.The blotters should be quite damp,but not soaking wet. 10.Rotate 90° counter clockwise.Electrophorese at pH 3.5,1.6 KV,13 min in the second dimension. The above electrophoresis times are appropriate when you are using a Salk/TVL/MBVL type electrophoresis rig
RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue
设备 1. Microcentrifuge capable of 13,000 x g 2. Sterile 0.5 mL microcentrifuge tubes 3. Ice bath 4. Microfuge tube pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen. Storage at -70°C
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