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文献和实验AFLP: not only for fingerprinting, but for positional cloning
for "personalizing" the preamplification and AFLP-PCR steps to fit ones needs.] Pre-amplification cocktail (recipe is for one rxn) 0.5 µL EcoRI-oligo.1 (@ 50 ng/µL) 0.8 µL 5 mM dNTPs 2.0 µL 10x-Promega Taq buffer [500 mM KCL, 100 mM Tris-HCl, pH 9.0 (@ 25
in magnetic stand for 30 sec or longer to collect the beads. c) remove supernatent and wash 3X with 200 ul of 0.5X SSC d) finally resuspend the SA-PMPs in 100ul of 0.5X SSC for each 200 ul of beads originally removed - each 100 ul aliquot
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
) is 1mg/ml in150mM NaCl, 50% glycerol, store at ©20C. Try 3 different [s] ofDNAse mix (A,B,C) 1,2 & 3ul stock DNAse1 2 ul 1M MgCl2 ©> 100ul H2O 4)binding rxn: components titrated & optimized by gel shiftassays 2ul probe mix Xul
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