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Generation of Recombinant Multimeric Antibody Fragments for Tumor Diagnosis and Therapy
in using bacterial expression systems is the high yield and low cost, especially when the VH and VL domains are tethered by a single-chain linker (scFv). This chapter describes expression systems for production of new types of scFv molecules
PCR amplification of variable regions (SOE-Protocol)
wherby the amplified VH and VL are connected by a primer encoded (Gly4Ser)3-linker. For the amplification of the variable regions, three different murine primer sets can be used: VH front (MuPD 3, 4, 5, 6, 7. 8. 9. 10, 11 and 12, mix) and VH back (MuPD
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