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文献和实验Flow Cytometry Assay to Measure Internalization of GPCRs
onto 6-cm dishes.2) Fill dishes with 2 mL growth media with 30 mM HEPES, pH 7.4. Set aside a dish of 293/B2AR at 4degC. You will use this dish to estimate the total number of receptors on the surface of a sample (i.e. before agonist treatment). HEPES reduces
Flow Cytometry Assay to Measure Internalization of GPCR's
media with 30 mM HEPES, pH 7.4. Set aside a dish of 293/B2AR at 4degC. You will use this dish to estimate the total number of receptors on the surface of a sample (i.e. before agonist treatment). HEPES reduces exposure of the cells to extreme pH values
Flow Cytometry Assay to Measure Internalization of GPCR
at 4degC. You will use this dish to estimate the total number of receptors on the surface of a sample (i.e. before agonist treatment). HEPES reduces exposure of the cells to extreme pH values outside of the incubator. 3) Add agonist to dishes and place
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