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文献和实验Monoclonal Antibody Production
. (7) Centrifuge samples at 10,000g 10 min. (8) Pipet off the serum supernatant.Store at -20 ℃.This is your pre-bleed control. Hybridoma Fusion 2. For one fusion you will need (1) 8500 ml bottles of DMEM media (2) 3500 ml bottles of fetal calf serum (FCS
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
monoclonal antibody production fusion
), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC = Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC
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