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文献和实验Technovit® 9100 Methyl Methacrylate
100 ml Masson's solution 20 ml Azophloxin solution 880 ml 0.2 % acetic acid For Massons solution mix 1 part of Masson's solution A with 2 parts of Massons solution B. Massons Solution A: 1 g acid
Extraction of DNA using DNAzol® Reagent
the culture plate and gently pipet the lysate into an assay tube. Cell Pellets or Suspensions: Add 1 ml of DNAzol® Reagent to 1-3 × 107 cells, either in pellet or in suspension (volume 100 µl), pellet the cells and wash them with 0.9% NaCl. Pellet
standard dilutions of BSA of 25, 50, 75 and 100 µ g/ml. Prepare appropriate serial dilutions of the sample to be measured. Place 1.0 ml of each of the above into separate tubes. Add 100 µ l of sodium deoxycholate to each tube.
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